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Feeding orange pulp improves bone microarchitecture in orchidectomized rats
Author(s) -
Morrow Rori,
Deyhim Farzad,
Patil Bhimanagouda S.,
Stoecker Barbara J.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a573-d
Subject(s) - pulp (tooth) , orange (colour) , antioxidant , osteoporosis , orchiectomy , endocrinology , medicine , zoology , chemistry , dentistry , food science , biology , biochemistry
This study evaluated effects of enhancing antioxidant status on bone quality in a rat model of osteoporosis. One‐year‐old retired breeder rats were randomly assigned to orchidectomy (ORX) or sham‐surgery (SHAM) treatments. Three days post‐surgery, ORX rats were assigned to the following treatments: ORX, ORX + 2.5% powdered orange pulp, ORX + 5.0% and ORX + 10% orange pulp. These isonitrogenous, isocaloric modified AIN‐93M diets with equal fiber content were pair fed for 4 months to the mean intake of SHAM. At the end of the study, blood and bone were collected. Plasma antioxidant capacity was determined spectrophotometrically and bone microarchitecture was assessed using microcomputed tomography. With a significant F statistic by ANOVA, the least square means procedure in SAS (version 9.1) was used to separate treatment differences (p<0.05). Lack of testosterone decreased (p<0.05) antioxidant status while feeding orange pulp as low as 2.5% maintained antioxidant capacity of ORX rats to that of SHAM. In 4 th lumbar trabecular cores, ORX rats had significantly reduced bone volume fraction, connectivity density, and trabecular number and increased trabecular separation. Five percent orange pulp tended and 10% orange pulp significantly increased (P<0.05) bone volume fraction, trabecular number, and decreased trabecular separation compared to SHAM. (Supported by USDA Grants 2001‐52102‐02294, 2004‐34402‐14768, TAMUK Research Council & Okla. Agricultural Expt. Station).