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Effect of isoflavones on lipid peroxidation and antioxidant status in mouse liver
Author(s) -
Ibrahim Wissam H.,
Chow Ching K.,
Bruckner Geza G.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a573-a
Subject(s) - lipid peroxidation , glutathione peroxidase , ascorbic acid , malondialdehyde , chemistry , antioxidant , glutathione , isoflavones , superoxide dismutase , vitamin e , medicine , endocrinology , biochemistry , food science , biology , enzyme
The purpose of this study was to determine the effect of isoflavones on lipid peroxidation and antioxidant status. Weanling C57BL6 mice were fed a basal diet (AIN‐93G) supplemented with either 1.08 gram isoflavone isolate/kg diet (0.25 g genistein/kg diet and 0.19 g diadzein/kg diet) or not supplemented (control) for 60 days. Immediately following sacrifice liver was processed for measuring the levels of the lipid peroxidation products malondialdehyde (MDA) and conjugated dienes (CD) as well as the levels of the antioxidants vitamin E, glutathione (GSH), ascorbic acid, catalase, selenium‐dependent glutathione peroxidase (Se‐GSH‐Px), selenium‐nondependent glutathione peroxidase (non‐Se‐GSH‐Px), and superoxide dismutase (SOD). Compared with the control group, mice fed the diet supplemented with isoflavones had significantly (P < 0.05) lower hepatic levels of MDA and CD. The levels of vitamin E, GSH, ascorbic acid and the activities of catalase, Se‐GSH‐Px, non‐Se‐GSH‐Px, and SOD were not altered by isoflavones supplementation. The results obtained provide experimental evidence that isoflavone supplementation confers protection against peroxidative damage to membrane lipids and does not alter hepatic antioxidant systems.