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Protein Expression Profiling Analysis of Human Breast Cancer Cells Treated with Genistein
Author(s) -
Kim Jong Sang,
Kim Jang Hoon,
Lim Hyun Ae,
Jang Chan Ho,
Kim Ju Ryung,
Sung Mi Kyung
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a565-c
Subject(s) - genistein , isoflavones , heat shock protein , mechanism of action , chemistry , biochemistry , biology , endocrinology , gene , in vitro
Genistein, one of soy isoflavones, have drawn much attention as a potential chemopreventive agent. Although the mode of action of genistein has been extensively studied, its precise mechanism for cancer prevention has remained elusive. To address the possible anti‐carcinogenic mechanism of genistein, we examined the protein expression pattern in breast cancer cells incubation with and without genistein using 2‐dimensional gel electrophoresis, MALDI‐TOF MS and an NCBinr database search. Genistein modulated the expression of several heat shock protein and various proteins related to diverse metabolic pathways. The regulated proteins were also involved in stress response, protein metabolism and modification, cell cycle and structure, glycolysis, nucleic acid metabolism, mRNA processing, and other cellular metabolism. Protein expression profile may contribute to revealing the mechanism of various biological activities of genistein.