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Modulation of interleukin‐6 (IL‐6) expression and secretion in adipose tissue in vitro and in vivo by n‐3 fatty acids
Author(s) -
Kim Hyojung,
Whelan Jay,
Allen Kenneth GD,
Pestka James,
Claycombe Kate
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a559-b
Subject(s) - adipose tissue , medicine , endocrinology , 3t3 l1 , eicosapentaenoic acid , secretion , chemistry , in vivo , adipose tissue macrophages , stromal cell , polyunsaturated fatty acid , fatty acid , biology , white adipose tissue , adipogenesis , biochemistry , microbiology and biotechnology
Obesity is associated increased serum interleukin‐6 (IL‐6) levels. Adipose tissue has been shown to secret up to 35% of serum IL‐6. However, only 10% of adipose tissue‐derived serum IL‐6 levels are accounted by the fully differentiated adipocytes. Thus, we previously tested and showed that adipose tissue stromal vascular cells and preadipocytes contribute significantly toward obesity‐induced increases in plasma IL‐6 levels. In our current study, we tested anti‐inflammatory effects of n‐3 polyunsaturated fatty acids (n‐3 PUFA) including eicosapentaenoic acid (EPA, 20:5) and EPA precursor stearidonic acid (SDA, 18:4) on 3T3‐L1 preadiopcytes and 3T3‐L1 adipocytes. Results showed that EPA (120 μM, 86% decrease) and SDA (120 μM, 82% decrease) significantly reduced lipopolysaccharide (LPS, 200 ng/ml)‐induced IL‐6 secretion in 3T3‐L1 preadiopcytes. Results also showed that EPA (120 μM, 24% decrease) and SDA (120 μM, 39% decrease) significantly reduced LPS‐induced IL‐6 secretion in 3T3‐L1 adipocytes. Further, we showed that n‐3 PUFA significantly reduced adipose tissue mesenchymal stem cell IL‐6 secretion in a dose dependent manner. These data suggest that n‐3 PUFAs have potent anti‐inflammatory effects on adipose tissue cell derived IL‐6 secretion.