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Effect of Niacin‐Bound Chromium Complex (NBC) on IL‐6 Secretion and Oxidative Stress Caused by High‐Glucose (HG) in Cultured U937 Monocytes.
Author(s) -
Jain Sushil K,
Rains Justin,
Rogier Kimberly
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a559-a
Subject(s) - oxidative stress , u937 cell , medicine , secretion , endocrinology , chemistry , insulin , insulin resistance , antioxidant , lipid peroxidation , cytokine , biochemistry , apoptosis
The molecular mechanism by which Cr3+ supplementation increases insulin sensitivity and decreases cardiovascular disease (CVD) is not known. Using U937 monocytes in a cell culture model, this study demonstrates that NBC inhibits the secretion of IL‐6, cytokine known to inhibit the sensitivity and action of insulin and risk of CVD. U937 cells were cultured with Control (6 mM) and HG‐medium (35 mM) in the absence and presence of NBC, CrCl3 or Cr‐picolinate (Cr‐P) (0.5‐10 uM) at 37oC for 24 hours. Cells stimulated with LPS. IL‐6 determined by sandwich ELISA, oxidative stress by lipid peroxidation. Data (n=4) show a significant stimulation of IL‐6 secretion and increase in oxidative stress in cells treated with HG. The effect of HG on IL‐6 secretion was 65% abolished by NBC, and to a lesser extent by CrCl3 (45%) and Cr‐P (21%). Effect of HG on oxidative stress was 100% inhibited by NBC, and CrCl3, but not by CrP. This demonstrates that Cr3+ mitigates the increases in IL‐6 and oxidative stress levels caused by the HG in cultured U937 cells. This provides evidence for a novel molecular mechanism by which Cr3+ supplementation may increase insulin sensitivity and reduce CVD in diabetes. In addition, these results show that the form of chromium affects efficacy and that NBC was more effective than CrCl3 and Cr‐P in this cell culture model (Supported by NIDDK and the Office of Dietary Supplements grant # RO1 DK064797).