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Dose‐response study of purified lycopene on biomarkers of oxidative stress
Author(s) -
Basu Arpita,
Devaraj Sridevi,
Cross Carroll E,
Meyer Stuart,
Jialal Ishwarlal
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a549-c
Subject(s) - lycopene , oxidative stress , dna damage , comet assay , chemistry , placebo , medicine , lipoprotein , endocrinology , pharmacology , biochemistry , carotenoid , cholesterol , dna , pathology , alternative medicine
Oxidative stress plays a pivotal role in the etiology of atherosclerosis and cancer. Tomato products containing lycopene, have been shown to be potent antioxidants, though, there exists no data on purified lycopene in humans. This is the first dose‐response, randomized, placebo‐controlled trial, testing the effects of purified lycopene supplementation on biomarkers of oxidative stress. Mildly hypercholesterolemic adults {n=77, (15‐20 per group), age ≥ 40 years}, consumed a lycopene‐restricted diet for 2 weeks, and were then randomized to receive 0, 6.5, 15, or 30 mg lycopene/ day for 8 weeks. Blood and urine samples were collected at the beginning and end (baseline) of 2 weeks of lycopene‐restricted diet, and, at 6 and 10 weeks of the study. Plasma lycopene levels were determined using reverse‐phase HPLC, lipoprotein oxidizability with copper ions, lymphocyte DNA damage by Comet assay, and urinary F 2 ‐isoprostanes by ELISA. Independent of the dose, plasma lycopene levels significantly increased in lycopene supplemented groups versus placebo (p< 0.05). ANOVA revealed a significant interaction effect for DNA damage. There was a significant 8.9% decrease in comet tail lengths at 8 weeks versus baseline with 30 mg lycopene/day (p= 0.007). No significant inter‐ or intra‐group differences were noted for low‐density lipoprotein (LDL) lag time and oxidation rate, or urinary F 2 ‐isoprostanes at any dose/time point (p> 0.05). Thus, purified lycopene supplementation was bioavailable and well‐tolerated by all subjects, and was shown to decrease cellular DNA oxidative damage, but, had no effects on other biomarkers of oxidative stress. (Roche)