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Interaction Between Murine Mesenchymal Stem Cells And T‐Cell Proliferation: Role Of Interleukin 1 Receptor Antagonist
Author(s) -
Pandey Amitabh C,
Dutreil Maria F,
Phinney Donald G
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a532-b
Subject(s) - mesenchymal stem cell , biology , microbiology and biotechnology , cell growth , in vivo , interleukin , cytokine , immunology , genetics
The objective of this study was to characterize the expression of interleukin 1 receptor antagonist (IL‐1rn) in immunodepleted murine mesenchymal stem cells (IDmMSCs), and evaluate its biological activity in vitro . Previously, we catalogued the transcriptome of IDmMSCs by serial analysis of gene expression (SAGE) and found an abundance of tags corresponding to IL‐1rn, an anti‐inflammatory protein that antagonizes interleukin 1. IDmMSCs were isolated from bone marrow of FVB/n mice via immunodepletion. Screening an IDmMSC cDNA library by PCR confirmed expression IL‐1rn. Enzyme‐linked immunosorbent assays (ELISA) demonstrated that IDmMSCs secreted 1 ng/ml/cell of IL‐1rn protein (p<.01). Immunofluorescence staining further revealed that IL‐1rn expression was restricted to specific sub‐populations of IDmMSCs, and FACS analysis confirmed that 24% of IDmMSCs expressed the protein. An in vitro T‐cell proliferation assay demonstrated that IL‐1rn secreted by IDmMSCs inhibited T cell proliferation (p<0.01). The current studies demonstrate that sub‐populations of IDmMSCs express high levels of IL‐1rn, which by counteracting the effects of IL‐1 in vivo , may regulate T‐cell proliferation and bone metabolism. The findings also implicate that IL‐1rn expression contribute to the immuno‐modulatory effects of MSCs as demonstrated in the amelioration of injuries such as bleomycin‐induced lung injury.