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Genetically engineered naphthalene sensitive whole‐cell bacterial bioreporters for the study of bioavailability
Author(s) -
Mancuso Matthew Cian
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a519-b
Subject(s) - bioavailability , naphthalene , bioreporter , genetically engineered , chemistry , biology , pharmacology , reporter gene , biochemistry , organic chemistry , gene , gene expression
Naphthalene is an important contaminant, and the primary ingredient in coal tar. It is poorly soluble, and a good model for other hydrophobic organic contaminants. The objective of this research was to study the role of complex matrices in mitigating bioavailable naphthalene. Biosensor bacteria were exposed to samples of sediments, chosen by the E.U. Abacus study on bioavailability for having various chemical properties, and controls all of which were subjected to various levels of naphthalene. Results were obtained using epi‐fluorescence microscopy and computer analysis. The data obtained showed a good correlation between the projected results as determined by chemical analysis, and expectations based on the chemical nature of the matrices. This project demonstrates the ability of these bioreporters to be used in a quantitative manner to study bioavailability of naphthalene in a vast array of complex matrix samples, and could be extended to samples exhibiting active biological dynamics impacting bioavailability.