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Effects of Lactobacillus F19 and breastfeeding on antibody responses to Haemophilus influenzae type B, diphteria‐ and tetanus toxoids
Author(s) -
West Christina,
Hernell Olle
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a5-d
Subject(s) - medicine , antibody , tetanus , placebo , haemophilus influenzae , immunization , immunology , vaccination , microbiology and biotechnology , antibiotics , biology , alternative medicine , pathology
Objective To investigate the effect of Lactobacillus F19 (LF19) from 4‐13 months of age on antibody responses to Haemophilus influenzae type B (Hib), diphteria‐ and tetanus toxoids (DT and TT). Methods In a double blind, placebo controlled study infants were randomized to cereals with (n=89) or without (n=90) addition of 1x10 8 CFU of LF19 per serving. They were immunized with Infanrix®‐Polio+Hib at (3), 5½ and 12 months of age. Antibodies to Hib and TT were measured by EIA (Enzyme immunoassay) and antibodies to DT by serum neutralization test in Vero cells. Stool samples were analyzed for LF19 with randomly amplified polymorphic DNA (RAPD)‐PCR. Results 84 and 87 infants completed the study in the LF19‐ and placebo‐groups, respectively. 54/84 infants were colonized with LF19 the entire intervention period. These infants and all infants in the placebo‐group were evaluated by a repeated measures ANOVA adjusting for breastfeeding < 6 months or ≥6 months (BF<6 mo or BF ≥ 6 mo). There was an interaction of LF19 and breastfeeding; LF19 and BF<6 mo augmented IgG antibody titres to DT and TT during immunization compared to placebo but there was no effect of LF19 in infants BF ≥ 6 mo (p<0.05). BF<6 mo enhanced IgG antibody titres to Hib during immunization compared to BF ≥ 6 mo (p<0.05) with no effect of LF19. Summary In infants BF<6 mo LF19 augmented antibody titres to DT and TT. There was no effect of LF19 in infants BF 6 mo. BF<6 mo augmented antibody titres to Hib compared to BF 6 mo with no effect of LF19. Conclusions Our results suggest that the duration of breastfeeding affects antibody response to Hib and influences the effects of probiotics on humoral immune response. The study was supported by Semper AB, Sweden, The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning and The Swedish Agency for Innovation Systems.