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RV2985 DIADENOSINE POLYPHOSPHATASE FROM M. TUBERCULOSIS, AN “INVASION” ENZYME HOMOLOG AND POTENTIAL NOVEL ANTIBIOTIC TARGET
Author(s) -
Ramos Julian,
Sheibley Daniel,
Glick Sarah,
O’Handley Suzanne
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a472-a
Subject(s) - enzyme , tuberculosis , antibiotics , pathogen , microbiology and biotechnology , nucleoside , human pathogen , biology , bacteria , cleave , biochemistry , genetics , medicine , pathology
The Nudix hydrolases are a family of enzymes that cleave substrates containing a nucleoside diphosphate linked to some moiety, x, and are identified by the common signature sequence: GX5E7REUXEEXGU, where U= I, L, or V. We have been systematically discovering and characterizing Nudix hydrolases from M. tuberculosis to identify potential novel antibiotic targets. One such enzyme, Rv2985 diadenosine polyphosphatase, is a homolog to enzymes shown to be responsible for the invasiveness of pathogenic bacteria. We are purifying and characterizing Rv2985 as a possible candidate for the M. tuberculosis “invasion” enzyme. Blocking the ability of a pathogen such as M. tuberculosis to be able to invade its human host may be an excellent target for the development of new antibiotics.

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