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Chromosome folding levels dependent and independent on gene expression
Author(s) -
Nurminsky Dmitry I.,
Mikhaylova Lyudmila M.,
Boutanaev Alexander M.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a469-b
Subject(s) - chromatin , nuclear matrix , gene , scaffold/matrix attachment region , biology , gene expression , genetics , chromosome , gene cluster , interphase , regulation of gene expression , chromatin remodeling , transcription (linguistics) , chia pet , microbiology and biotechnology , linguistics , philosophy
Spatial organization of chromatin in the interphase nucleus plays an important role in gene expression. We found an explicit relationship between intranuclear localization of various chromosome segments and the pattern of gene distribution along the genome sequence. The relatively gene‐poor ca. 0.3 Mb chromosome segments interact with components of the nuclear matrix to form compact stable cluster(s) that probably comprise the “backbone” of the chromosome territory. The gene‐rich segments also form clusters which are dependent on non‐matrix nuclear proteins and apparently represent the active transcription neighborhoods. The resulting composite structure formed by clusters of gene‐rich and gene‐poor regions seems to be independent of the tissue specific pattern of gene expression and therefore reflects the stable rather than the dynamic aspect of nuclear architecture. This suggests that chromatin remodeling that occurs in individual chromatin domains to accomodate transcriptional activation or repression of particular genes does not extend to the higher order chromatin structures.