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Transcriptional program of Singapore grouper iridovirus
Author(s) -
Chen Liming,
Hew Choy Leong
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a466-d
Subject(s) - iridovirus , grouper , orfs , biology , gene , gene expression , virus , genetics , virology , open reading frame , fishery , fish <actinopterygii> , peptide sequence
Grouper iridovirus is a cause of serious systemic diseases, which result in the significant economic losses in aquaculture. In order to take more insights into grouper iridovirus and provide the fundamental basis for the further researches on this group of virus, we examined simultaneous expression measurements of nearly all predicted open reading frames (ORFs) in Singapore Grouper Iridovirus (SGIV) by using the viral DNA microarray and uncovered the SGIV transcriptional program. Of the 127 viral elements on the SGIV array, 13% genes commenced expression at 1hpi, including ORF086L which is a putative immediate‐early gene; 83% genes commenced expression at 4hpi, including the ORF006R which is a putative early gene; 4% genes commenced expression at 8hpi, including the ORF072R which is a Major Capsid Protein‐late gene. And 53.5% viral genes were detected to reach maximum expression at 32hpi; 34% viral genes were detected to reach maximum expression at 48hpi; 1.5% viral genes were detected to reach maximum expression at 72hpi; 11% viral genes were detected to reach maximum expression at 96hpi. Our results may provide an important basis for understanding mechanisms driving the grouper iridovirus temporal cascade. This work was financially supported by the grant “Ministry of Education (MOE)” to Choy Leong Hew.