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In Vitro Study of Breast Cancer Cells Exposed to Antisense of Liver Fatty Acid Binding Protein
Author(s) -
Miller StacyAnn,
Waddy Byron,
Chakraborty Nabarun,
Hammamieh Rasha,
Jett Marti
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a463
Subject(s) - fatty acid binding protein , fatty acid synthase , breast cancer , apoptosis , fatty acid , adipose tissue , cell , cancer cell , in vitro , chemistry , microbiology and biotechnology , cancer , biology , biochemistry , gene , genetics
Fatty acids are important for energy delivery and synthesis of membrane lipids and lipid mediators. We have confirmed the altered expression level of different FABPs on breast cancer cells, the most common form of malignancy in women. Liver fatty acid binding protein (L‐FABP) and Intestine fatty acid binding protein (I‐FABP) were reported to be up‐regulated in breast cancer cell lines while adipose and epidermal fatty acid binding protein (A‐FABP and E‐FABP) were down regulated in cancer cells compared to normal cell lines. In this study we examined the expression levels of FABPs in MCF‐7 breast cancer cells that were grown in the presence of phosphorothioate oligodeoxynucleotides, which were commercially synthesized, then in the presence of L‐FABP antisense or sense ODNs. Total RNA were isolated from the cells, the quality and concentration was evaluated and then analyzed using fluorescent cDNA microarray technology. Real‐Time Polymerized Chain Reaction was then carried out to confirm the change in expression levels in certain genes. Changes in expression levels of four FABPs were noted. These expression patterns show that LFABP initiate apoptosis and suppress growth and metastasis in cells.