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Cryptococcus neoformans cell cycle control and proteomic analysis
Author(s) -
Kawamoto Susumu,
Virtudazo Eric V,
Ohkusu Misako,
Yamanaka Yuko,
Hirano Hisashi,
Takeo Kanji
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a460-b
Subject(s) - cryptococcus neoformans , microbiology and biotechnology , cryptococcus , biology , computational biology
Very recently (Science 307: 1321‐1324, 2005), completion of the genome sequencing project of the pathogenic yeast Cryptococcus neoformans has just reported. C. neoformans has emerged as a major pathogenic microbe in patients with impaired immunity such as AIDS patients. Like the model yeast Saccharomyces cerevisiae, C. neoformans is also a budding yeast but it exhibits some cell cycle characteristics different from S. cerevisiae. In C. neoformans, during exponential growth, budding and DNA synthesis occur together, but as the growth phase progresses during late log to early stationary phase, budding is delayed towards the end of the cell cycle at G2 phase, allowing cells to arrest at either G1 or G2 unbudded state. As a first step to elucidate the mechanisms for C. neoformans cell cycle molecularly, we isolated the C. neoformans homologue of CDC28/Cdc2 (cyclin‐dependent kinase, Cdk‐1) molecule, the main cell cycle gene which regulates the major processes in eukaryotic cell cycle. We also have been cloning other genes regarding C. neoformans cell cycle control. In order to further analyze the control mechanism of C. neoformans cell cycle molecularly at the protein level, we have been performing the proteomic analysis of the yeast cells.