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Role of YidC in the Insertion and Folding of Membrane Proteins
Author(s) -
Dalbey Ross Edgar
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a457
Subject(s) - protein subunit , protein targeting , atp synthase , membrane protein , microbiology and biotechnology , membrane , chemistry , biology , biochemistry , biophysics , enzyme , gene
The YidC/Oxa1p/Alb3 family of proteins is a new class of proteins that play a role in the membrane assembly of proteins in bacteria, mitochondria and chloroplasts. In 2000, YidC was shown to be an essential protein for bacterial growth. YidC plays a direct role in the membrane insertion process and is absolutely essential for the membrane insertion of the Sec‐independent M13 procoat and Pf3 coat protein. Recent studies show that YidC plays an important role for the insertion of certain energy transduction proteins. YidC can promote the insertion of the F 0 sector subunits of the ATP synthase. The ATP synthase inserts by the novel YidC pathway, while subunits a and b appear to insert by the Sec/YidC pathway. In addition, YidC plays an important role for subunit II (cyoA) of the respiratory protein cytochrome bo oxidase, which is made with a cleavable signal peptide and spans the membrane twice with a short N‐terminal domain and a large C‐terminal domain. We find contrasting requirements for insertion of the N‐terminal and C‐terminal domain. Namely the amino‐terminal domain of CyoA inserts by the YidC only pathway whereas the C‐terminal domain inserts by the Sec/YidC pathway. This work was supported by NIH grant GM63862‐05.