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Osmotic resistance of the erythrocytes as a marker of adaptation to hypobaric hypoxia
Author(s) -
Dobrovolskiy Gennady A.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a448-b
Subject(s) - cytolysis , hypoxia (environmental) , hypobaric chamber , medicine , endocrinology , chemistry , andrology , biology , oxygen , biochemistry , anatomy , cytotoxic t cell , in vitro , effects of high altitude on humans , organic chemistry
It was established that osmotic resistance of the erythrocytes (ORE) is increased under the influence of hypoxia with any significant correlation with the phases of adaptation. White nonlinear 180–250g rats (52) were subjected for 6 hours a day to hypobaric hypoxia in the chamber (560 mm Hg) for 21 days. Control animals (50 rats) were kept under the normal atmospheric conditions. Blood measurements from the tails were performed by 1, 7 and 21 days. We analyses the data by program complex “Cytolis”. Complete erythrocyte cytolysis (CYC) was detected by 21 st day of hypoxic influence (p< 0.01) with remarkable upregulation during the first day (p< 0.05). The time of 50% cytolysis (T50%L) and ORE was not changed. Time frame of cytolysis was decreased during due to hypoxia (p< 0.05). CYC was inhibited only on the first day of hypoxia (p< 0.05); while weak responses were registered within the experimental time (p< 0.01). ORE was up regulated during the first (urgent) phase of adaptation, demonstrated down regulation by day 7 (transition phase) and was normalized by day 21. The number of share low‐firm erythrocytes (LFE) was not changed with hypoxia compare with control, while the share mean‐firm erythrocytes (MFE) were significantly decreased (p<0.05). Share high‐firm erythrocytes (HFE) demonstrated up‐regulation in urgent phase, down regulation in adaptive phase, but did not show normalization by day 21. Conclusion Established ORE, CYC and MFE changes due to prolonged hypobaric hypoxia could be recognized as a sensor in adaptation for hypobaric hypoxia, important for sportsman and clinical resuscitation care [HL 79675].