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Vasoactive intestinal polypeptide receptors (VIP1 and VIP2) are morphogenic modulators of embryonic coronary vessel tube formation
Author(s) -
Dedkov Eduard I,
Christensen Lance P,
Tomanek Robert J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a440-c
Subject(s) - quail , receptor , explant culture , stimulation , chemistry , vasoactive intestinal peptide , blockade , in vitro , medicine , microbiology and biotechnology , endocrinology , biology , biochemistry , neuropeptide
This study tested the hypothesis that selective activation or blockade of VIP1 and VIP2 receptors will affect embryonic coronary vessel tube formation. The in vitro quail heart explant model was used. The ventricular apices from the hearts (stage HH29) were placed on collagen I gel and cultured for 48 hr in the medium with 10% FBS. For the next 48 hr, the explants were treated with the VIP receptor selective agonists (1 nmol/μL) and/or antagonists (10 nmol/μL). The explants were fixed and immunolabeled with the quail‐specific anti‐endothelium antibody. The total length of tubulo‐like outgrowth (TLT) was quantified using ImagePro Plus software. In each explant, TLT was normalized by the explant perimeter and, finally, TLT in treated explants was expressed as a percent from TLT in untreated controls (UC). The concurrent stimulation of VIP1 and VIP2 induced only an 8% increase in TLT, but when VIP1 and VIP2 was activated independently the TLT was increased by 32% and 24%, respectively (P<0.05 vs. UC). The simultaneous blockade of VIP1 and VIP2 inhibited TLT by 29% (P<0.01 vs. UC), but a selective blockade of VIP1 alone reduced TLT only by 18% (P<0.01 vs. UC). Our data demonstrate that the interaction between VIP1 and VIP2 receptors exerts positive as well as negative modulatory effects on embryonic coronary vessel tube formation. Supported by NIH RO1‐HL62587.