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Effects of lactic acid bacteria cultures with enhanced levels of GABA on immune cell stimulation
Author(s) -
Park KiBum,
Kim NamSuk,
Oh ChanHo,
Oh SukHeung
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a431-c
Subject(s) - immune system , lactic acid , stimulation , biology , cell culture , pharmacology , microbiology and biotechnology , chemistry , bacteria , biochemistry , immunology , endocrinology , genetics
Lactic acid bacteria (LAB) having high γ‐aminobutyric acid (GABA)‐producing capacity were isolated from naturally aged fermented foods. Immune cell‐stimulating activities of the LAB cultures were measured. The levels of GABA in the cultures of LAB‐A and LAB‐B were 3,082 nmole/mL and 58 nmole/mL, respectively. Subpopulations of murine splenic B and T cells increased significantly by LAB‐A treatments. The LAB‐A treatments increased B and T cells up to 26%, and Th cells up to 24%. However, the subpopulations of thymocytes Th and Tc cells were not changed significantly by the LAB treatments. Interleukin 2 (IL‐2) and γ‐Interferon (IFN‐γ ) production in mice serum increased up to 95% and 33% by the LAB‐A treatments, respectively. Nitric oxide also increased significantly in LAB‐A treated RAW264.7 macrophage cell lines. The LAB‐A retarded significantly the proliferation rates of U937 promonocytic and HL60 acute promyelocytic leukemia cells. However, LAB‐B does not stimulate immune cells. These results suggest that the cultures of the LAB‐A were effective for the improvement of immunoregulatory action, possibly due to the enhanced levels of GABA and/or combined effects of several components including GABA. (Supported by grants from BioGreen21 program of Rural Development Administration to S. H. Oh).