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Effect of kiwifruit extract on antioxidant activity and erythrocyte membrane fluidity in rats
Author(s) -
Ma AiGuo,
Zhang Yan,
Han XiuXia,
Lan Jin,
Gao YiHuai
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a429-a
Subject(s) - membrane fluidity , microviscosity , antioxidant , chemistry , ascorbic acid , membrane , biochemistry , food science
Backgroud Kiwifruit is known to be enriched in ascorbic acid and various other antioxidant components. The purpose of our study was to investigate the effects of kiwifruit extracts on antioxidant activity and erythrocyte membrane fluidity in rats. Methods Seventy Wistar rats (aged 4 months, 250–260g BW) were randomly divided into 5 groups (n=14). The first group was a control; the second, third, fourth and fifth groups were supplemented with 5%, 10%, 15% and 30% kiwifruit extracts. After the 30‐day trial, membrane fluidity was measured by the fluorescence polarization and MDA and SOD in rat erythrocyte membranes were measured. Results The .OH induced by the Fenton reaction in the rat erythrocyte membranes caused a significant increase in MDA and a decrease in SOD activity. However, there were significantly lower levels of MDA in groups receiving 15% and 30% kiwifruit extract supplements (P<0.01), and higher SOD activity in the 30% kiwifruit extract group, compared to the control group (P<0.05). The fluorescence polarization (P) and microviscosity (¦Ç) of erythrocyte membranes increased when oxidative damage was induced induced by .OH, but the kiwifruit extract supplements decreased P and ¦Ç values and enhanced the fluidity of erythrocyte membranes in groups receiving 15% and 30% kiwifruit extracts (P<0.05). Conclusion In this study, 15% to 30% kiwifruit extract supplement prevented chemical‐induced oxidative damage and enhanced the fluidity of erythrocyte membranes in rats. This suggests a kiwifruit extract offers beneficial antioxidants effects in vivo. Granted by Institute of Natural Drugs, New Zealand