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Activation of Extracellular‐Signal Regulated Kinase (ERK1/2) by stochastic resonance is Ca2+ dependent in MC3T3‐E1 osteoblasts
Author(s) -
Li Jiliang,
Stockwell David W.,
Simmons Christopher R.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a417-b
Subject(s) - extracellular , extracellular signal regulated kinases , stochastic resonance , kinase , chemistry , signal (programming language) , microbiology and biotechnology , biophysics , resonance (particle physics) , biochemistry , protein kinase a , biology , physics , computer science , noise (video) , particle physics , artificial intelligence , image (mathematics) , programming language
Stochastic resonance, the same type of vibration encountered during exercise, enhances osteoblastic response to mechanical stimulation. This study aimed to explore the role of calcium channels in osteoblastic response to stochastic resonance. MC3T3‐E1 osteoblastic cells were exposed to the following conditions: (1) Sine – an oscillating sinusoidal fluid flow at 2 Hz (a peak shear stress of 12 dynes/cm 2 ), (2) Sine+Vibration – an oscillating sinusoidal fluid flow at 2 Hz of a decreased amplitude (a peak shear stress of 10 dynes/cm 2 ) in combination with broad frequency vibrational components of 0–50 Hz (1.2 dynes/cm 2 of the mean amplitude) to invoke stochastic resonance, or (3) Control – no exposure to fluid flow stimulation. Exposure to sine + vibration fluid shear elicited a significantly larger increase in activation of ERK1/2 over sine wave fluid shear alone. However, blocking the mechanosensitive cation‐selective channels with administration of gadolinium inhibited the activational effect of vibration on ERK1/2. Inhibiting intracellular Ca 2+ release by administering thapsigardin also significantly decreased the activation of ERK1/2 induced by vibration. Furthermore, inhibition of the entry of calcium through the L‐type voltage sensitive calcium channels with nifedipine significantly attenuated the osteoblastic response to both the sine wave and sine+vibration fluid shear stress. These data suggest that osteoblast sensitivity to stochastic resonance is mediated by Ca 2+ . This study is supported by NASA grant NNA04CD04G.