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Reaction Pathways and Stoichiometry of Nitric Oxide Synthase
Author(s) -
Gao Yingtong,
Ishimura Yuzuru,
Panda Satya P.,
Marohnic Christopher C.,
Martásek Pavel,
Bettie Sue S.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a41-a
Subject(s) - chemistry , reagent , nitric oxide synthase , stoichiometry , nitric oxide , substrate (aquarium) , oxygen , reactive oxygen species , cysteine , photochemistry , atp synthase , catalase , nitrous oxide , enzyme , combinatorial chemistry , biochemistry , organic chemistry , oceanography , geology
Nitric Oxide Synthase (NOS) is known to catalyze more than one reaction. The NO producing reaction is recognized as the coupled reaction, and the uncoupled reactions are the ones that produce reactive oxygen species (ROS), such as H 2 O 2 and/or O 2 − . Our detection of nitrous oxide (N 2 O) as a product of NOS makes the reaction pathways more complicated, and many parallel/sequential events are subject to further research. Quantitative tools, such as a fluorescence based oxygen probe, headspace GC/MS and UV‐Vis spectroscopy are incorporated to do simultaneous measurement of various substrate/products; and trapping reagents, such as hemoglobin, L‐cysteine and catalase are implemented to push‐and‐pull the reactions to different pathways. Our results show that NOS simultaneously catalyzes multiple reactions, which can be more or less favorable under certain conditions.