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Exercise‐induced increases in mRNA’s encoding oxidative enzymes in human skeletal muscle late in recovery
Author(s) -
Leick Lotte,
Damsgaard Rasmus,
Grønlykke Lars,
AlAbaiji Fatma,
Saltin Bengt,
Pilegaard Henriette
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a389-c
Subject(s) - oxidative phosphorylation , citrate synthase , messenger rna , gene , enzyme , gene expression , medicine , endocrinology , biology , skeletal muscle , biochemistry
Training‐induced increases in oxidative enzymes have been suggested to steam from cumulative effects of transient gene responses to each single exercise bout. However, mRNA changes are not detected for genes encoding oxidative enzymes within the initial 8h of recovery from an exercise bout. To test the hypothesis that genes encoding oxidative enzymes are up‐regulated in the late part of recovery, eight male subjects completed 90 min of cycling exercise (70% VO2max). Muscle biopsies were obtained from the vastus lateralis muscle before exercise, and after 10h, 14h, 18h and 24h of recovery. The mRNA content was determined for selected genes and normalized to single stranded cDNA content. Citrate synthase mRNA was up‐regulated (p<0, 05) 4 fold at 18h of recovery, whereas carnitine palmitoyl transferase I mRNA, cytochrom c mRNA and hormone sensitive lipase mRNA exhibited (p<0, 05) biphasic increases with 2–3 fold at 10h of recovery and 2–4 fold at 18h of recovery. However, significant changes were not detected in 3‐hydroxy‐acyl‐dehydrogenase mRNA. The present data demonstrate that genes encoding central oxidative enzymes are indeed up‐regulated at the mRNA level late during recovery from a single exercise bout supporting that transient exercise‐induced gene responses can be an underlying mechanism leading to training‐induced increases in mitochondrial enzymes. The study was supported by grants from The Danish Natural Science Council, The Danish Medical Research Council and the Novo Nordisk Foundation.