Premium
Effect of Geldanamycin upon HSP 70 content in skeletal muscle
Author(s) -
Farrar Roger P.,
Choe Judy,
Hammers David,
Bowman Phillip,
Walters Thomas J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a386-a
Subject(s) - geldanamycin , skeletal muscle , chemistry , medicine , endocrinology , hsp70 , heat shock protein , hsp90 , biochemistry , gene
HSP 70 content of skeletal muscle is elevated following 6 weeks of endurance training. Protection against ischemia/reperfusion damage to skeletal muscle is also provided by endurance training (Merritt et al, 2005). The goal of this study was to determine whether HSP 70 levels could be elevated in skeletal muscle in sedentary animals without endurance training or elevated body temperature. Geldanamycin has been demonstrated to inhibit HSP 90 and to induce HSP 70 in a number of tissues and organs, however its affects in skeletal muscle are not documented. This study was designed to determine the time course and dosage of geldanamycin administration to induce HSP70 in skeletal muscle. Male Sprague Dawley rats, 350 g, were given 0.25g, 1.0, or 2.5 μg/g body weight and euthanized 6 hrs later. The Plantaris (PLN) and Soleus (SOL) muscles were removed and evaluated for HSP 70 content. The PLN constitutively expresses HSP 70 at approximately 25% of the levels of the SOL. Administration of 1.0 and 2.5 μg/g demonstrated 300 and 400% elevation in HSP 70 in the PLN and 45 and 100 percent in the SOL. The time course for induction of HSP 70, following i.p. administration of 2.5μg of geldanamycin per gram of body weight, was determined at 6, 12, 24, and 72 hours. The HSP 70 levels peaked at 12 hours. These data provide evidence that geldanamycin may provide pharmacological protection against tourniquet‐induced ischemia /reperfusion.