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Heating enhances muscle regrowth rate and reduces oxidant stress
Author(s) -
Selsby Joshua Taylor,
Rother Sara,
Tsuda Shige,
Pracash Om,
Quindry John,
Dodd Stephen
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a385-d
Subject(s) - skeletal muscle , chemistry , antioxidant , soleus muscle , medicine , endocrinology , heat shock protein , muscle atrophy , sarcopenia , oxidative stress , biochemistry , gene
Muscle regrowth after disuse is characterized by a period of increased oxidant stress. We have previously shown that heating can act as an antioxidant therapy. We hypothesized that heating during reloading would induce heat shock proteins resulting in an increased rate of skeletal muscle regrowth and reduced oxidant stress when compared to reloading alone. Rats were divided into four groups: control (Con; n=10), immobilized (Im; n=10), reloaded control (R7C; n=10), and reloaded heated (R7H; n=10). One week of immobilization resulted in a 50% and 90% reduction in HSPs 25 and 72, respectively, and an ~50% induction of HSP 32 while reloading returned HSPs to Con levels. HSP content was increased ~30% in the R7H group when compared to the R7C group. Immobilization resulted in a 45% reduction in soleus muscle mass. Reloading did not significantly increase soleus muscle mass but the R7H group recovered 50% of the loss caused by atrophy. Oxidant damage to lipids was increased 20% and 25% while protein oxidation was increased 60% and 80% in the Im and R7C groups compared to Con, but was reduced to Con levels in the R7H group. CuZn SOD activity was increased ~30% and Cat activity was increased ~20% in Im and R7C compared to Con, while the R7H was similar to Con. We conclude that heating during reloading increases the rate of skeletal muscle regrowth through a reduction in oxidant stress independent of predominant antioxidant enzymes. (Supported by the NFLPA)