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Co‐localization of FosB and cFos in the supraoptic nucleus (SON) of dehydrated male rats
Author(s) -
Garza Jacob C.,
Martinez Michelle A.,
Gottlieb Helmut B.,
Cunningham J. Thomas
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a359-a
Subject(s) - dehydration , fosb , chemistry , medicine , endocrinology , andrology , zoology , biology , biochemistry , transcription factor , gene
Previous studies have shown that water deprivation significantly increases cFos and FosB staining in the SON. The purpose of this study was to determine if cFos and FosB are expressed in the same cells following dehydration and rehydration. Adult male rats were water deprived for 48 h or 46 h with 2 h access to water prior to perfusion. Controls had ad libitum access to water. Following perfusion, forebrain sections were stained using a cFos antibody raised in rabbit (Oncogene AB‐5) and a FosB antibody raised in goat (Santa Cruz, sc‐48‐g) with Cy3 conjugated donkey anti‐rabbit and Cy2 conjugated donkey anti‐goat secondary antibodies (Jackson ImmunoReseach). Stained sections containing the SON were examined using a laser scanning confocal microscope. Sections that were incubated with one primary antibody and both secondary antibodies showed appropriately homogenous green or red staining. In the SON, dehydration was associated with significant increases in cFos labeling as compared to control, while rehydration was not different from control (cFos: control 1 ± 0.3; dehydration 32 ± 3; rehydration 2 ± 1). FosB staining in the SON was significantly increased following dehydration and rehydration as compared to control (FosB: control 1 ± 0.5; dehydration 36 ± 4; rehydration 32 ± 6). Following dehydration, 93% of the cFos positive cells were also labeled for FosB, while control and rehydration had 6% and 22% respectively. The results demonstrate a high degree of co‐localization of cFos and FosB in the SON following dehydration. Supported by HL62579 & DK57822