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Quercetin and NPPB‐induced diminution of aldosterone action on Na+ absorption and ENaC expression in renal epithelium
Author(s) -
Fujimoto Shinichiro,
Niisato Naomi,
Sugimoto Tohru,
Marunaka Yoshinori
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a347-a
Subject(s) - epithelial sodium channel , bumetanide , aldosterone , endocrinology , medicine , chemistry , transepithelial potential difference , cotransporter , ion transporter , biology , sodium , biochemistry , organic chemistry , membrane
In renal epithelial A6 cells, aldosterone applied for 24 h increased the transpithelial Cl− secretion due to activation of the Na+/K+/2Cl− cotransporter (NKCC), and stimulated the transepithelial Na+ absorption, activity of epithelial Na+ channel (ENaC) and alpha‐ENaC mRNA expression. The stimulatory action of aldosterone on the transepithelial Na+ absorption, ENaC activity and alpha‐ENaC mRNA expression was diminished by 24 h‐pretreatment with quercetin (an activator of NKCC) or NPPB (a blocker of Cl− channel), while 24 h‐pretreatment with bumetanide (a blocker of NKCC) enhanced the stimulatory action of aldosterone on transepithelial Na+ absorption. On the other hand, under the basal (aldosterone‐unstimulated) condition, quercetin, NPPB or bumetanide had no effects on transepithelial Na+ absorption, activity of ENaC or alpha‐ENaC mRNA expression. These observations suggest that although aldosterone shows overall its stimulatory action on ENaC (transepithelial Na+ transport), aldosterone has an inhibitory action on ENaC (transepithelial Na+ transport) via activation of NKCC, and that modification of activity of Cl− transporter/channel participating in the transepithelial Cl− secretion influences the aldosterone‐stimulated ENaC (transepithelial Na+ transport). Supported by Grants‐in‐Aid from JSPS (17390057, 17590191).