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Chromatin remodelers pump DNA around the nucleosome
Author(s) -
Cairns Bradley Robert,
Wittmeyer Jacqueline,
Saha Anjanabha
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a34-c
Subject(s) - nucleosome , chromatin , dna , genetics , biology , computational biology
A central question in chromatin biology is how chromatin remodeling complexes (remodelers) use the energy of ATP hydrolysis to move nucleosomes along DNA. The enzymatic component of the remodeler RSC is Sth1, which we have shown is an ATP‐dependent DNA translocase. On DNA substrates, RSC/Sth1 tracks along one strand of the duplex with a 3′→5′ polarity and a tracking requirement of one base, properties that may enable the directional translocation of DNA along the surface of the nucleosome. Sth1 binds the histone octamer and also binds nucleosomal DNA approximately two DNA turns from the nucleosome dyad. Results with various nucleosome substrates suggest that RSC/Sth1 remains at this fixed position on the histone octamer, and Sth1 conducts directional DNA translocation from this position, drawing in DNA from one side of the nucleosome and pumping it toward the other. Additional results suggest that RSC/Sth1 provide access to nucleosomal DNA primarily through translocation of DNA to positions outside the nucleosome. This work is supported by the Howard Hughes Medical Institute and the National Institutes of Health (GM60415).