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Kidney gene expression in diabetes pre‐ and post‐puberty
Author(s) -
Lane Pascale Hammond,
Langer William J
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a334-a
Subject(s) - ctgf , endocrinology , medicine , growth factor , diabetes mellitus , kidney , renal function , insulin , litter , insulin like growth factor , renal hypertrophy , connective tissue , muscle hypertrophy , biology , diabetic nephropathy , receptor , pathology , agronomy
Many kidney diseases accelerate during puberty, including diabetes mellitus (DM). Rats with experimental DM induced prior to puberty show less renal and glomerular hypertrophy over 6 weeks than those with adult onset. Transforming growth factor β appears to be differentially regulated; however, it is likely that other genes are as well. Male littermates had one member of each litter assigned to each of these groups: Young Control, Young DM, Adult Control, and Adult DM. Young groups received streptozocin, 65 mg/kg IV, or vehicle at 4 weeks of age. Adult groups received similar treatment at 14 weeks of age. Insulin pellets were placed 3 days after injection to maintain blood glucose 300‐400 mg/dl for 6 weeks. Under isoflurane anesthesia the kidneys were removed and snap‐frozen in liquid nitrogen. Once all animals had completed the protocol, RNA was isolated and analyzed with a commercial array including more than 28,000 genes. Data were analyzed by ANOVA. Differences ≥1.7‐fold with p<0.05 were considered significant; 4,278 genes met these criteria. Known profibrotic growth factors were differentially regulated, including connective tissue growth factor (CTGF). Primers were designed and real time RT‐PCR used to confirm age‐specific upregulation of CTGF in DM, and CTGF protein was assessed via immunoblotting.Many biological processes are differentially regulated in response to DM pre‐ and post‐puberty in the male rat, including CTGF. Further study will likely result in new directions for experimentation at the level of the gene, protein, and integrated function.