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Pathophysiological Increase in Atrial Natriuretic Peptide (ANP) Decreases Gastric Motility in Mice
Author(s) -
Addisu Anteneh,
Gower William R.,
Landon Carol S.,
Dietz John R.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a332-c
Subject(s) - atrial natriuretic peptide , pathophysiology , motility , medicine , natriuretic peptide , endocrinology , biology , heart failure , microbiology and biotechnology
Impaired gastric motility occurs in several pathophysiological states where plasma levels of natriuretic peptides are elevated. Thus, we studied the direct effect of natriuretic peptides on gastric motility. C57BL/6 mice were anesthetized with sodium pentobarbital. Catheters were placed in the trachea, carotid artery and jugular vein. Intragastric Pressure (IGP) was continuously monitored using a transducer attached to a balloon catheter. Following a 1 hour equilibration period the mice received either ANP (10ng/g iv) in 100μl of modified Krebs buffer or 100μl of Krebs buffer alone (vehicle). Differences between peak and baseline IGP were averaged and compared for three periods: basal (0‐30 min), injection (10‐40 min) and recovery (90–120 min) after respective injections. ANP significantly (p<0.05) decreased IGP from 2.12 ± 0.42 mmHg (basal) to 0.71 ± 0.24 (injection). IGP returned to 2.45 ± 0.39 during recovery (p<0.05). There was no significant change in the vehicle group where IGP was 0.85 ± 0.12, 0.94 ± 0.30 and 0.97 ± 0.33 mmHg for the respective periods. Average gastric contractions per minute were 4.4 ± 0.7, 3.1 ±0.4 and 4.3 ± 0.5 for the three periods of ANP injection compared to 5.3 ± 1.2, 4.2 ± 0.6 and 4.3 ± 0.5 for the vehicle group (all p>0.05). The results show that ANP decreases gastric motility without significantly affecting contraction rate. (Support: V. A. Merit Award to WR Gower and NSF IGERT grant 0221681)