Premium
Yeast lipidomics: Transcriptional and metabolic regulation in response to inositol
Author(s) -
Henry Susan A.,
Gaspar Maria L.,
Aregullin Manuel A.,
Jesch Stephen A.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a33-c
Subject(s) - inositol , endoplasmic reticulum , lipid metabolism , phosphatidylinositol , phospholipid , saccharomyces cerevisiae , lipidomics , microbiology and biotechnology , biology , biochemistry , metabolism , phosphatidylcholine , signal transduction , gene expression , yeast , gene , membrane , receptor
Addition of inositol to the growth medium induces a rapid and profound alteration in the pattern of synthesis and turnover of the major membrane phospholipids in the yeast, Saccharomyces cerevisiae . Simultaneous genome‐wide changes occur in the expression of several distinct sets of genes which respond to signals emanating from the endoplasmic reticulum (ER) (see Abstract 799, S. Jesch and S. Henry), the major site of phospholipid synthesis. To gain insight into potential signals responsible for these transcriptional patterns, we conducted a kinetic analysis of the changes in lipid composition and metabolism and gene expression following the addition of inositol. We now report that the introduction of inositol results in a rapid increase in the total cellular content of phosphatidylinositol (PI) accompanied by a rapid decrease in phosphatidylcholine (PC) content. Moreover, changes in the acyl chain length and degree of unsaturation among the various PC species were also observed. These alterations in PI and PC metabolism, in turn, generated altered patterns of utilization of their immediate precursors. The relationship of altered lipid metabolism and the correlated changes in transcription will be discussed in terms of potential signaling mechanisms. Overall, these results suggest that alterations in membrane lipid metabolism produce acute effects on global gene expression patterns and that the interplay among several distinct endoplasmic reticulum membrane‐localized signaling pathways contributes to this response. This work is supported by NIH Grant GM‐19629.