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Kv1.3 channel redistribution in the immunological synapse varies according to the T lymphocyte activation state
Author(s) -
Nicolaou Stella A.,
Szigligeti Peter,
Neumeier Lisa,
Conforti Laura
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a326-a
Subject(s) - immunological synapse , cd28 , microbiology and biotechnology , colocalization , t cell , biophysics , chemistry , confocal microscopy , cd3 , antigen , biology , immunology , cd8 , t cell receptor , immune system
T lymphocyte and antigen presenting cell contact triggers the formation of the immunological synapse (IS), a signalosome important for T cell activation. Kv1.3 channels participate in T cell activation by modulating Ca2+ influx in resting but not in activated (pre‐exposed to antigen) T cells. Further, Kv1.3 channels have been shown to localize in the IS (Panyi et al., PNAS 2004). Despite the key role of these channels in T cell activation, their behavior during IS formation is not fully understood. Herein we characterized the kinetics of Kv1.3 channel recruitment into the IS in resting and activated human T cells. IS formation was induced by 1–30min exposure to either anti‐CD3/CD28 antibody coated beads or EBV infected B cells. Cells were immunostained for Kv1.3 and F‐actin and imaged with fluorescence and confocal microscopy. We found that binding of the CD3/CD28 beads to both T cell types leads to colocalization of Kv1.3 with F‐actin (marker of IS) at the bead/cell contact area. Kv1.3 redistribution depends on IS formation, as it was not observed with control beads. Further, recruitment of Kv1.3 channels in the IS in resting T cells builds up progressively, with maximal recruitment at 30min. In contrast, activated T cells show maximal Kv1.3 recruitment at 1min and by 15min the channels move out of the IS. These results were confirmed using B cells. In conclusion Kv1.3 channel redistribution in the IS follows a characteristic time course depending on the T cell activation state. This difference may reflect the different role that Kv1.3 channels play in these cells. (NIH‐CA95286 to LC).