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Adult stem cells and angiogenic factors for myocardial infarction
Author(s) -
Carter Jeffrey E.,
Hossler Fred E.,
Li Chuanfu,
Kao Race L.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a315-b
Subject(s) - ligation , medicine , angiogenesis , stromal cell , myocardial infarction , stem cell , cardiology , infarction , mesenchymal stem cell , pathology , biology , microbiology and biotechnology
The goal of this study is to use bone marrow stromal cells and angiogenic factors to induce myogenesis and angiogenesis after myocardial infarction. Inbred Lewis rats were used for marrow stromal cell isolation. Rats were randomly assigned into four treatment groups (10 per group): 1). Sham; 2). Ligation; 3). Ligation + Cell; and 4). Ligation + Cell + Angiogenic Factors. At two weeks after left anterior descending coronary artery ligation, serum free culture medium was injected into the area of infarction for the Ligation group, medium with labeled stromal cells for the Ligation + Cell group, and cells transfected with VEGF165 and Ang‐1 plus labeled cells (at 50:50 % ratio) were used for the 4th group. Six weeks after initial surgery, cardiac function was assessed using a Millar pressure conductance unit. Left ventricular pressure‐volume loops were recorded after release of vena cava occlusion to calculate the slope for Emax. By setting the control group (Sham) as 100%, the Emax slopes of group 2 = 54 ± 5%; group 3 = 96 ± 13%; and group 4 = 86 ± 22%. Pathological evaluations also showed significant thinning and scaring of the Ligation group as compared to all other groups. Stem cell implantation into the myocardial infarction significantly improved ventricular function and induced myogenesis at the sites of implantation. Including stem cells expressing angiogenic factors did not further improve the outcome. This may be due to the significant inflammatory response induced by adenoviral factors. When angiogenesis was quantified by corrosion casting, marked increases in vascularization were observed in the cell implantation groups. Supported by NIH grant HL72138 and AHA grant 0255009B