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Differential expression and function of dopamine receptor subtypes in kidney and mesentery artery of ecSOD knockout mice
Author(s) -
wang xiaoyan,
Solis Glenn,
Welch William J,
Wilcox Christopher S,
Jose Pedro A
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a307-a
Subject(s) - receptor , knockout mouse , kidney , biology , medicine , endocrinology
Dopamine receptors (DR) regulate blood pressure, in part, by inhibiting the production of reactive oxygen species (ROS). To determine whether increased ROS production affects DR protein expression, we studied in ecSOD knockout (ecSOD−/−) mice and wild type littermates (ecSOD+/+), protein expression (immunoblotting) of the five DRs in the kidney and relaxation response in isolated mesentery artery to DR agonists: D 1 R/D 5 R(Fenoldpam), D 2 R(PPHT), D 3 R(PD128907) and D 4 R(PD168077). ecSOD−/ − (n=6) and ecSOD+/+ littermates (n=6) (male, 4–6 months) were fed 0.4% Na diet with ad libitum water and food intake. In the whole kidney, D 1 R protein expression was similar in ecSOD−/ − and ecSOD+/+ (ecSOD+/+: 100±14, ecSOD−/ − mice: 85±15%, expressed as M± E percentage of ecSOD+/+, set at 100%). D 5 R protein at 55kDa was also similar in the two mouse strains but the 70 kDa band (possibly glycosylated receptor) was decreased in ecSOD−/ − (47±6 %) relative to ecSOD+/+ (100±20%). Compared to ecSOD+/+, all D 2 ‐like receptors were decreased in ecSOD−/ − (D 2 R: 49±9; D 3 R: 28±6; and D 4 R: 82±3%). Relative to ecSOD+/+, relaxation of isolated mesentery arteries caused by D 3 R and D 4 R agonists was decreased in ecSOD−/ − but the relaxation response to D 1 /D 5 R and D 2 R agonists was similar in ecSOD−/ − and ecSOD. Furthermore, D 4 R protein was also decreased in ecSOD−/ − (61%) while D 5 R was similar in the two mouse strains. The decreased renal protein expression of D 2 R, D 3 R, D 4 R, and D 5 R and decreased D 3 R and D 4 R vascular protein expression and relaxation caused by ROS contribute to the dysregulation of blood pressure and renal function in ecSOD−/ − mice.