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Peripheral arteries take up but do not concentrate 5‐HT
Author(s) -
Ni Wei,
Lookingland Keith,
Watts Stephanie W.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a302-b
Subject(s) - pargyline , monoamine oxidase , aorta , incubation , medicine , endocrinology , peripheral , metabolite , serotonin , chemistry , basal (medicine) , monoamine neurotransmitter , biology , biochemistry , enzyme , receptor , insulin
Existence of 5‐hydroxytryptamine (5‐HT) and a functional 5‐HT transporter were reported in peripheral arteries. The existence of 5‐HT in arteries was confirmed by observing 5‐HT staining in freshly dissociated aortic smooth muscle cells. We hypothesized that peripheral arteries takeup and store 5‐HT. By using HPLC, we measured basal levels of 5‐HT and its monoamine oxidase A (MAOA) metabolite 5‐hydroxyindolacetic acid (5‐HIAA) released to physiological salt solution (PSS, data not shown) and that which remained in aorta after 4 or 8 hours PSS incubation. 5‐HT (1 uM, 15 min incubation) was also preloaded in aorta from normal or pargyline (MAOA inhibitor)‐treated rats. Naïve aorta from normal rats lost 5‐HIAA with time while 5‐HT concentration remained at basal level ( Table 1). In 5‐HT preloaded aorta, aorta kept 5‐HT concentration low by metabolism and releasing 5‐HIAA in normal rats and by releasing 5‐HT in pargyline‐treated rats ( Table 2). Thus, arteries can regulate circulating 5‐HT by taking up and metabolizing but not storing 5‐HT. As changes in circulating 5‐HT concentrations were reported in diseases such as hypertension, arterial regulation of 5‐HT concentration is important to understand. AHA 0415397 1.2.