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Effects of ethanol and ethyl pyruvate on adhesive events for human endothelial or lung epithelial cells and neutrophils
Author(s) -
Johansson AnneSofie,
Palmblad Jan
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a290
Subject(s) - umbilical vein , a549 cell , tumor necrosis factor alpha , in vivo , in vitro , pharmacology , stimulation , chemistry , endothelial stem cell , inflammation , immunology , medicine , biochemistry , biology , microbiology and biotechnology
Ethanol (ethyl alcohol, EtOH), administered in vivo or in vitro, is associated with reductions of host defense systems, including functional responses of neutrophils, endothelial and epithelial cells. Although this anti‐inflammatory effect of EtOH points to a potential as a therapeutic agent, well known side effects limits the clinical use of EtOH. Instead, ethyl pyruvate (EtP) has recently emerged as an inexpensive immunomodulatory alternative. Here, we sought to compare effects of EtP and EtOH on adhesive events in and between human neutrophils, umbilical vein endothelial (HUVEC) and lung epithelial (A549) cells in vitro. When fMet‐Leu‐Phe activated neutrophils were treated with EtOH modest impairments of adhesion to HUVEC were noted, whereas EtP conferred substantial reductions. Likewise, when HUVEC were stimulated by LPS, IL‐1β or TNFαafter treatment with 170 mM EtOH, we observed modest reductions of adherence, whereas 2.5–10 mM of EtP dose‐dependently abrogated adherence induced by all three agents. The surface expression of E‐selectin, ICAM‐1 or VCAM‐1 on the HUVEC, induced by LPS, IL‐1β or TNFαwere nor affected by EtOH but were markedly reduced by EtP. A substantial reduction of E‐selectin expression was noted when EtP was added two hours after LPS stimulation. Generation of cytokines of significance for adhesive and proliferative events of HUVEC, IL‐8 and G‐CSF, were also more potently impaired by EtP. In A549 cells similar reactions were observed. ‐ Thus, EtP impedes adhesive events in and between endothelial and lung epithelial cells and neutrophils of significance for early events in the inflammatory response.