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HSP100 and caspase‐3 expression in retinoic or irradiation induced apoptosis during early eye development.
Author(s) -
Gashegu Julien,
Vanmuylder Nathalie,
Duterre Mariam,
Rooze Marcel,
Louryan Stéphane
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a28-c
Subject(s) - apoptosis , caspase 3 , retinoic acid , biology , caspase , antibody , microbiology and biotechnology , andrology , immunology , programmed cell death , cell culture , biochemistry , medicine , genetics
Apoptosis is an essential physiological process in embryonic development. During eye development, two apoptosis periods can be distinguished: early and later periods. Within the apoptosis pathway, caspase‐3 plays a crucial role. It has also been shown that HSP100 may have potential role in apoptosis. The aim of this study was to show the relationship between HSP100 and caspase‐3 in physiological and induced apoptosis in early eye development. Methods Two strains of pregnant mice were used. C57Bl/6J mice received all‐trans retinoic acid (80mg/Kg), while NMRI mice were irradiated (2 Grays) on the 9 th gestational day. Embryos were harvested at 3, 6, 12 and 24 hours after exposition. Coronal sections were obtained. Staining using either anti caspase‐3 (the antibody is directed against caspase‐3 active form) or anti HSP100 immunohistochemistry was performed. Controls embryos of both strains were processed similarly. Results HSP100 and caspase‐3 expression presented similar temporo‐spatial pattern. The latter pattern depended on the exposition. After retinoic exposition, caspase‐3 and HSP100 positive cells were increased in optic vesicle. Whereas after irradiation, caspase‐3 and HSP100 positive cells were noticeably increased in optic vesicle, peri‐optical mesoderm and less in lens placode. The HSP100 expressed before caspase‐3.