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Does Vegf Mediate Pronephros Induction?
Author(s) -
Hejazi Homayoun,
Mauch Teri Jo,
Clough Lani,
Kourtides Demetrius
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a27
Subject(s) - pronephros , somite , biology , intermediate mesoderm , mesoderm , microbiology and biotechnology , embryo , medicine , endocrinology , anatomy , embryogenesis , gastrulation , biochemistry , embryonic stem cell , zebrafish , gene
Does Vegf mediate pronephros induction The methodology for observing the effect of Affi‐gel blue beads on the kidney pronephros of stage HH 6‐8 (approximately 23‐29 hours of incubation time) chick embryos is described. We’ve previously shown that somites are necessary and sufficient for pronephros induction. We hypothesize that resonant angioblasts within the somites might mediate this induction. The method consists of affixing one Affi‐gel blue bead, soaked in SU‐5614 or Vegf. Beads soaked in Vegf were implanted in the lateral mesoderm caudal to the first somite. Beads soaked in SU‐5614 were placed near prospective somite 6 and/or intermediate and lateral mesoderm. Embryos were cultured using the EC culture method. Different incubation times of 6, 12, and 24 hrs were used to optimize the effects of the addition of SU‐5614. Post culture, embryos were subjected to in situ hybridization using pax‐2 as a molecular marker for pronephros induction. If Vegf signaling is sufficient for pronephros induction, Pax‐2 expression should increase. SU‐5614 is a Vegf inhibitor and if it decreases pronephros induction it will block normal Pax‐2 expression, demonstrating that Vegf is required.