Premium
Nischarin as a functional imidazoline (I1) receptor
Author(s) -
Zhang Jian,
AbdelRahman Abdel A
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a252-a
Subject(s) - receptor , rilmenidine , transfection , imidazoline receptor , signal transduction , transmembrane protein , microbiology and biotechnology , biology , hek 293 cells , agonist , pharmacology , biochemistry , gene
Nischarin is the mouse homologue of human imidazoline receptor antisera‐selective (IRAS) protein, a candidate of the imidazoline I1 receptor. Tansfection of the IRAS into the mammalian cells expressed the binding sites of I1 receptor. Nischarin and IRAS share the functions of enhancing cell survival, growth and migration. Bioinformatics modeling indicates that the IRAS and Nischarin may be transmembrane proteins. The convergence information raised the interesting possibility that Nischarin may be the same protein as I1 receptor. To test this hypothesis, we developed anti‐Nischarin antibodies primarily produced according to the sequences of the Nischarin protein, and conducted I1‐ receptor signal transduction (functional) studies with the I1‐receptor agonist rilmenidine in the presence and absence of antisense oligodeoxynucleotides (ODNs) of Nischarin. NIH3T3 cells transfected with the Nischarine cDNA and incubated with the newly synthesized antibody expressed a 190 kd band. Under similar conditions, the PC12 cells expressed the Nischarin band around 210 kd, which is consistent with reported findings in other cells of neuronal origin; The immunoflourescence findings showed the targeted protein to be associated with the cell membrane in PC12 cells. Nischarin ODNs abolished the expression of the Nischarin in PC12 cells. Equally important, Nischarin ODNs abolished the phosphorylation of the MAPK p42/44 elicited by I1‐receptor activation (rilmenidine) in nerve growth factor (NGF) differentiated PC12 cells. Together, the present findings present evidence that Nischarin serves as the functional I1 receptor at least in the differentiated PC12 cells.