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Reduction of the drug seeking behaviour via up‐regulation of opioid receptors using sex steroids
Author(s) -
Vahabzadeh Abdolvahab,
Hidarieh N.,
Orian S
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a240-d
Subject(s) - opioid , morphine , (+) naloxone , opioid receptor , testosterone (patch) , endocrinology , saline , opioid antagonist , receptor , medicine , endogenous opioid , chemistry , pharmacology
Recently our studies focused on the neuropeptides including sex steroids. The present investigation aimed to use combination of naloxone and testosterone for induction of opioid up‐regulation in rats. Male Sprague‐Dawley rats (200–300g) were divided into 3 groups of control, sham, and experimental (n=10). The experimental group received single doses of naloxone and testosterone (S. C.) for 10 days. Same amount of normal saline also injected to the sham group for the same period of time. After 10 days all 3 groups exposed to both water and water with morphine (50mg/Kg). 5 min tail pinch is applied for 3 groups. The tail pinch‐induced behavioral responses were observed and monitored. In the present studies duration tail pinch responses was observed at 5 min. The experimental group was compared with the control and sham animals. Water and/or morphine preference were monitored for all groups. Variation of volume of the water and/or morphine conception in all groups calculated in the percentage base. All statistical analysis was carried out using absolute data and either student paired t‐test or Mann‐Whitney U nonparametric test. The result shows that the volume of the morphine conception under stress of tail pinch was 100% in both control and sham groups. In the experimental groups the volume reduced to 83+/‐09% (P<0.005, n=18) under the same stress. Data from the present studies suggest the naloxone as opioid antagonist induces up‐regulation of opioid receptors. This means that up‐regulation of opioid receptor reduce the drug seeking behavior even under the stress. This protocol going to be carries out in the humane.