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Comorbidity of depression with opioid addiction in the reserpinized rat
Author(s) -
Vahabzadeh Abdolvahab,
Hadipor A.,
Hydarieh N.,
Khorami A.,
Ghodarzi Z
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a240-c
Subject(s) - microdialysis , chloral hydrate , serotonergic , reserpine , anesthesia , morphine , neurochemical , buprenorphine , analysis of variance , chemistry , medicine , opioid , dopamine , serotonin , receptor
One line of research in our division involved in drug abuse. A minor genetic abnormality in one of brain neurochemical systems may consider as a factor in this issue (Vahabzadeh, A. and Khorasani, M. 2000). To complete the whole picture, the present studies aimed to investigate the role of defect in serotonergic system on vulnerability of addiction. Male Sprague‐Dawley rats (200–300g) were implanted with microdialysis probes in the Rafe area under chloral hydrate anesthesia. The implanted animals were perfused at 2 Micro L/min with Ringer solution 12 hours after implantation. In control group animals were placed in cage with access to food and water. In the sham group Ringer solution were perfused via microdialysis probes for 3 days and 8 hours per day. For the test group Reserpine (10 Micro M) was added to the Ringer solution for same period. Then all groups received morphine (50 mg/Kg) injection 3 times a day for another 3 days in white chamber, using conditional place performance (CPP) technique. The time period of conditioning was monitored in all groups of animals. Variation calculated in the percentage base. All statistical analysis was carried out using absolute data and either student paired t‐test (within same group) or ANOVA (within the different groups). Drug seeking behavior (CCP) enhanced by 57+/‐14% (P<0.005, n=7) in reserpinized group when compared to the control and/or sham group. These data suggest that lack of serotonergic system and/or depression may play an important role in addition, which our recent studies also confirm, the self treatment drug abuse in depression.