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The positive inotropic effect of ethanol extract of Lepidium apetalum in beating rabbit atria
Author(s) -
Choi Deok Ho,
Kim Seung Ju,
Kang Dae Gill,
Lee Ho Sub
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a234-b
Subject(s) - verapamil , inotrope , glibenclamide , staurosporine , medicine , diltiazem , chemistry , channel blocker , stroke volume , atrium (architecture) , endocrinology , pharmacology , blood pressure , heart rate , protein kinase a , calcium , atrial fibrillation , kinase , biochemistry , diabetes mellitus
In the present study, the positive inotropic effects of ethanol extract of Lepidium apetalum (ELA) and possible mechanisms responsible for this effect were investigated in beating rabbit atria. ELA (5 x 10 −4 g/ml) significantly increased atrial stroke volume, pulse pressure, and cAMP efflux in perfused beating rabbit atria. These effects were not altered by pre‐treatment with diltiazem (5 x 10 −6 M) or verapamil (1 x 10 −6 M), L‐type Ca 2+ channel blockers. In addition, ELA‐induced increases in atrial stroke volume, pulse pressure, and cAMP were not inhibited by pre‐treatment of staurosporine (1 x 10 −6 M), a non‐specific protein kinase inhibitor. ELA markedly increased K + concentration in beating atria‐derived perfusate. Helveticoside (2 x 10 −5 M), a well known digitalis‐like cardiac glycosidic constituent of ELA, also increased atrial stroke volume and pulse pressure without changes in cAMP efflux, which were partially inhibited by pre‐treatment with diltiazem, verapamil, or staurosporine, respectively. These results suggest that ELA‐induced positive inotropic effect in beating rabbit atria might due to digitalis‐like activity of helveticoside and partially be related with the increase in cAMP.