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Antileukemic Effect of a Novel Nutrient Mixture on Human Jurkat T Cells
Author(s) -
Roomi M. Waheed,
Ivanov Vadim,
Niedzwiecki Aleksandra,
Rath Matthias
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a224-b
Subject(s) - jurkat cells , matrigel , zymography , fetal bovine serum , matrix metalloproteinase , microbiology and biotechnology , trypan blue , chemistry , gelatinase , in vitro , ascorbic acid , in vivo , cell culture , cancer cell , biochemistry , biology , immunology , medicine , cancer , t cell , immune system , genetics , food science
Matrix metalloproteinase (MMP) expression and production are associated with advanced stage tumor and contribute to tumor progression, invasion and metastasis. A unique non‐toxic nutrient mixture (NM) consisting of lysine, proline, ascorbic acid and green tea extract has been shown to exhibit anti‐tumor activity against a number of cancer cell lines both in vivo and in vitro . Objective In this study we sought to examine the effect of NM on human acute Jurkat T cells on proliferation, MMP expression and invasion through Matrigel. Methods and Materials Human T Jurkat cells (ATCC) were grown in RPMI‐1640 medium with 10% fetal bovine serum and antibiotics and treated with NM at 0, 10, 50, 100, 500 and 1000 μg/ml concentration in triplicate at each dose. Cells proliferation was assessed by counting cells stained with Trypan blue, invasion was evaluated through Matrigel, and MMPs by gelatinase zymography. Cells were also treated with PMA to induce MMP‐9 activity. Results NM was not toxic to cells at 100 μg/ml concentration and exhibited antiproliferative effect at 500 μg/ml concentration. Zymography demonstrated a faint band corresponding to MMP‐9 and enhanced activity after PMA stimulation. NM showed a dose dependent inhibition in MMP‐9 activity with virtual total inhibition at 500 μg/ml concentration. Matrigel invasion was significantly reduced at 500 μg /ml and totally blocked at 1000 μg/ml NM. Conclusions Our results demonstrate that NM is a promising new therapeutic agent for acute leukemia, and is potential candidate for human trials.