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In vivo metabolism of dopamine is altered by brain iron deficiency: a model for RLS
Author(s) -
Bianco Laura E,
Beard John L
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a193-a
Subject(s) - microdialysis , dopamine , endocrinology , in vivo , medicine , extracellular , striatum , chemistry , circadian rhythm , biology , biochemistry , microbiology and biotechnology
Alterations in dopamine receptor density, decreased DA transporter density and increased extracellular DA have been attributed to brain ID in Restless Legs Syndrome. In vivo microdialysis with no net flux was used to assess the basal extracellular concentration of DA in the striatum of ID rats. Extracellular DA was increased in the striatum of ID animals, but the extraction fraction (no net flux approach) did not differ between ID and control animals. A second experiment examined the impact of IDA on the circadian rhythm of monoamine metabolism through in vivo microdialysis. In the transition between light and dark, the ID animals displayed elevated DA prior to this transition as compared to controls, but there was no shift however in the diurnal cycle. The final experiments tested the hypothesis that L‐DOPA treatment in RLS may be affected by brain ID. Administration of carbidopa/L‐dopa caused a significantly larger increase in NE in IDA rats compared with control rats. These in vivo experiments support the claims of alterations in dopamine biology in brain iron deficiency.