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Proliferation and tissue organization of human ES cell‐derived cardiomyocytes
Author(s) -
Johkura Kohei,
Cui Li,
Ogiwara Naoko,
Sasaki Katsunori
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a18-d
Subject(s) - microbiology and biotechnology , cell growth , in vitro , embryonic stem cell , myocyte , transplantation , biology , proliferating cell nuclear antigen , cellular differentiation , cell , stem cell , in vivo , medicine , biochemistry , genetics , gene
Cardiomyocytes developed from human embryonic stem (ES) cells are expected to be useful for cell therapy in heart diseases. We assessed the proliferation and differentiation of ES cell‐derived cardiomyocytes in vitro , and their ability to organize tissues in extracardiac sites. Differentiation of the cardiomyocytes from early to advanced stages was detectable during the culture of EB outgrowths. Structures involved in function of cardiomyocyte differentiated with distinct immunoreactivity for constituent proteins/peptides. Proliferation potential was maintained in part of those cardiomyocytes for more than 30 days after EB expansion judging from their PCNA immunoreactivity. Twenty eight days after transplantation of EB outgrowths into the retroperitoneum of adult SCID mice, various types of cardiomyocyte‐rich tissue had been formed in teratomas with a varied density of cardiomyocytes among non‐cardiac cells. The morphological features of cardiomyocytes were also diverse, while their proliferation potential was generally low. These results showed that human ES cell‐derived cardiomyocytes differentiate in vitro , and survive in vivo forming cardiomyocyte‐rich tissues. The failure in organizing authentic myocardial tissues is thought to be mainly due to their low proliferation potential in extracardiac sites, which suggests the need of a way to accelerate or maintain their proliferation.