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Colonic Gene Expression Profiles of Young Adult and Geriatric Dogs Fed Different Diets
Author(s) -
Vester Brittany Marie,
Apanavicius Carolyn J.,
Swanson Kelly S.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a172-a
Subject(s) - weanling , biology , gene expression , transcriptome , gene , gene expression profiling , medicine , endocrinology , zoology , genetics
The objective of this study was to measure gene expression differences of colon tissue of healthy young adult and geriatric dogs fed two distinct diets. Six geriatric (11 yr‐old at baseline) and 6 weanling (8 wk‐old at baseline) female beagles were fed one of two diets for 12 months: 1) Animal‐protein based diet (APB) containing 28% protein, 23% fat, and 5% dietary fiber; or 2) Plant‐protein based diet (PPB) containing 26% protein, 11% fat, and 15% dietary fiber. RNA, isolated from colon mucosal scrapings was hybridized to Affymetrix GeneChip Canine Genome Arrays. After array normalization, data were analyzed using the mixed models procedure of SAS. Transcripts having a P < 0.05 and > 1.5‐fold change were considered different. Age affected gene expression level in dogs fed the PPB (354 transcripts) and APB (173 transcripts) diets. Crypt depth was greater in old dogs. Similarly, many genes associated with cell cycle regulation and signal transduction were upregulated in old dogs vs. young dogs. Old dogs also had decreased expression of detoxification enzymes. Diet*age interactions were also observed, including several genes associated with nutrient and ion transport, cell‐cell signaling and cell adhesion. This research was funded in part by the National Center for Supercomputing Applications and the University of Illinois, under the auspices of the NCSA/UIUC Faculty Fellows Program, and by Pyxis Genomics, Inc.