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Diet Composition Impacts Skeletal Muscle Gene Expression Profiles of Young Adult and Geriatric Dogs
Author(s) -
KarrLilienthal Lisa Kathleen,
Apanavicius Carolyn Josephine,
Swanson Kelly Scott
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a172
Subject(s) - skeletal muscle , weanling , sarcopenia , gene expression , transcriptome , biology , medicine , endocrinology , gene , microarray , oxidative phosphorylation , physiology , genetics , biochemistry
Aging is associated with loss of skeletal muscle mass and increased oxidative damage. Diet may play a role in attenuating these changes. The objective of this study was to measure gene expression differences in skeletal muscle of healthy young adult and geriatric dogs consuming two distinct dog foods. Six geriatric (11 yr‐old at baseline) and 6 weanling (8 wk‐old at baseline) female beagles were randomly assigned to one of two diets: 1) Animal‐protein based (APB) containing 28% protein, 23% fat, and 5% dietary fiber; or 2) Plant‐protein based (PPB) containing 26% protein, 11% fat, and 15% dietary fiber. Dogs remained on experiment for 12 months. RNA, isolated from skeletal muscle samples, was hybridized to Affymetrix GeneChip Canine Genome Arrays. Following microarray normalization, data were analyzed using the mixed models procedure of SAS. Transcripts having a P < 0.05 and > 1.5‐fold change were considered different. Diet manipulated gene expression in both geriatric (116 transcripts) and young adult (12 transcripts) dogs. In old dogs, APB diet appeared to be detrimental as it decreased expression of genes associated with muscle contraction, carbohydrate metabolism, and calcium and oxygen transport. This research was funded in part by the National Center for Supercomputing Applications and the University of Illinois, under the auspices of the NCSA/UIUC Faculty Fellows Program, and by Pyxis Genomics, Inc.