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Prophylactic enrichment of enterocyte phospholipids with polyunsaturated fatty acids fed to suckling piglets
Author(s) -
Hess Holly Aline,
Corl Ben A.,
Lin Xi,
Averette Gatlin Lori A.,
Phillips Oulayvanh T.,
Harrell Robert J.,
Blikslager Anthony T.,
Odle Jack
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a170-b
Subject(s) - enterocyte , polyunsaturated fatty acid , eicosapentaenoic acid , arachidonic acid , chemistry , phospholipid , fatty acid , food science , small intestine , docosahexaenoic acid , biochemistry , biology , zoology , enzyme , membrane
Infant formula companies began fortifying formulas with long‐chain polyunsaturated fatty acids in 2002, including arachidonic acid (AA) at ~ 0.5% of total fatty acids. This study is the first in a series that will examine whether supra‐physiological supplementation of AA will improve intestinal health. The objective of this study was to determine the time specific effects of feeding formula enriched with AA on fatty acid composition of enterocyte phospholipids and on the functional development of the small intestine. One day old pigs (N=96) were fed a milk‐based formula for 4, 8, or 16 d. Diets contained either no polyunsaturated fatty acids (0%AA, negative control), 0.5%AA, 2.5%AA, 5%AA, or 5% eicosapentaenoic acid (EPA) of total fatty acids. A sow‐fed reference group (N= 18) also was included. Growth (299 +/− 21 g/d) was unaffected by treatment (p>0.6). By day 16, ileal enterocyte phospholipid concentrations of AA were enriched 176%, 280%, and 355% in pigs fed the 0.5%AA, 2.5%AA, and 5%AA diets, respectively, compared with the 0%AA negative control. Concentration of AA within enterocytes of the 5% EPA fed pigs was similar to that of the 0%AA fed pigs, while EPA concentration increased by >8 fold. These data demonstrate a dose‐dependent response in enterocyte AA concentration to dietary AA and that supra‐physiologic supplementation of AA is not detrimental to growth. Supported by award 2005‐35200‐16174, USDA‐NRI, CSREES.