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Influenza infection disrupts glucose uptake in 3T3‐L1 adipocytes
Author(s) -
Harmon Anne W.,
Beck Melinda A.
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a163-c
Subject(s) - lipid raft , adipocyte , viral replication , glucose uptake , adipose tissue , phosphorylation , in vivo , biology , virus , microbiology and biotechnology , budding , glucose transporter , titer , function (biology) , influenza a virus , virology , insulin , endocrinology , signal transduction
Lipid rafts function as platforms for the entry, assembly, and budding of influenza virus. Because adipocytes have an abundance of lipid rafts, we questioned whether influenza could infect adipocytes and alter their function. We found that 3T3‐L1 adipocytes support viral replication: the titer 24 h after infection exceeded the inoculum dose by nearly tenfold. At this time, most infected cells remained viable but exhibited impaired glucose uptake and markedly reduced Akt phosphorylation. Collectively, these results highlight the usefulness of the adipocyte model for studying both viral replication and the mechanism by which viral infection inhibits insulin‐stimulated glucose uptake. In addition, this is the first description of an influenza virus infecting and replicating in adipocytes and may suggest that adipose tissue in vivo could be a target for viral infection. Research support: NIH P20RR20649