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Modulation of Bcl‐2 family and activation of caspase by linoleic acid in human gastric cancer cells
Author(s) -
Park KunYoung,
Kwon Jae Im,
Jung KeunOk,
Lee YoungEun,
Park Cheol,
Choi Young Hyun
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a156
Subject(s) - linoleic acid , apoptosis , dapi , carcinogenesis , cell growth , cancer cell , cell , biology , cancer , chemistry , cancer research , biochemistry , microbiology and biotechnology , fatty acid , genetics
Recent epidemiologic studies and animal model studies support a possible role for polyunsaturated fatty acids in prevention of carcinogenesis or inhibition cancer cell growth. However, the molecular mechanisms of their anti‐proliferative action on cancer cell growth are not known completely. In this study, we investigated the effects of linoleic acid on the cell proliferation of AGS human gastric cancer cells in order to understand its anti‐proliferative mechanism. MTT assay indicated that treatment of AGS cells to linoleic acidresulted in the inhibition of cell proliferation in a concentration‐dependent (50 uM ~ 200 uM) manner. The anti‐proliferative effect of AGS cells by linoleic acid was associated with apoptotic cell death as measured by flow cytometric analysis and DAPI staining. Linoleic acid treatment resulted in the up‐regulation of a pro‐apoptotic Bax expression and a concomitant inhibition of anti‐apoptotic Bcl‐2 expression. Additionally, linoleic acid treatment induced the proteolytic activation of caspase‐3, which is believed to play a central role in the apoptotic signaling pathway and degradation of poly(ADP‐ribose) polymerase and phospholipase C‐gamma 1 protein. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti‐cancer activity of linoleic acid.