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Gene‐ nutrition interactions controlling colon cancer development
Author(s) -
Gao Ying,
Soloway Paul
Publication year - 2006
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.20.4.a151-d
Subject(s) - colorectal cancer , grading (engineering) , biology , inbred strain , strain (injury) , physiology , medicine , cancer , gene , oncology , genetics , ecology
Studies suggest that colorectal cancers result from complex interactions between inherited genetic susceptibility and environmental factors. Identifying the genetic loci that control diet responsiveness may provide insights into nutritional based cancer intervention strategies. Recombinant inbred (RI) mice may be useful for the mapping of such loci. To validate the usefulness of RI approach, we tested the BXA F1 along with its parental strains, the C57BL/6 and the A/J, in an experiment that induced colon cancer in a diet‐dependent manner. Any indication of diet responsiveness shall warrant future use of RI approach for such mapping. C57BL/6J, A/J and BXAF1 males were subjected to feeding with either test diet (NWD) or control diet ( AIN‐76A) from 6 weeks of age for 35 weeks. They were given AOM i.p. weekly (10mg/kg) from 8 weeks of age for 8 weeks. 25 weeks following the last AOM injection, tumor counts, sizes and histological grading data were collected. Results showed that NWD feeding increased the average tumor counts per mouse for all three strains. This effect is statistically significant in BXAF1 strain. Notably, the average tumor counts in BXAF1 mice are significantly higher than that of its progenitors. Though strain effect resulted in significant differences in tumor size and histological grading, diet effect did not. The differences showed in tumor counts between strains in same diet and the differences between diets in same strain implied a gene‐nutrition interaction in colon tumor development in this study. Given the diet‐dependent tumor development is a complex quantitative trait, the results also suggest that the BXA RI panel may be of use for mapping of QTL involved in diet responsiveness of colon cancer development.